Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse ES cells)
Loading amount
50000 cells
Specification
mouse ES cells
Treatment
72 hours after esiRNA Knockdown
Gel Running Conditions
Non-reduced Denaturing (Invitrogen NuPage 4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/3000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% milk in PBS_0.1% Tween 20
Secondary antibody
Dilution
1/4000
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG (H+L) HRP conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 73 kDa
Positive control
Correct size (around 73 Kd), Very specific (only one band) and strong signal.
Clear decrease of band signal after MISSION® esiRNA knockdown against Cpsf73, compared to control samples
Clear decrease of band signal after MISSION® esiRNA knockdown against Cpsf73, compared to control samples
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Apr 21 2010