Anti-COX5A抗体[6E9B12D5] (ab110262)
Key features and details
- Mouse monoclonal [6E9B12D5] to COX5A
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2a
概述
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产品名称
Anti-COX5A抗体[6E9B12D5]
参阅全部 COX5A 一抗 -
描述
小鼠单克隆抗体[6E9B12D5] to COX5A -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Mouse, Rat, Cow, Human -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, and HepG2, Human embryonic lung-derived fibroblasts (strain MRC5), HeLa cells
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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纯化说明
ab110262 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
克隆
单克隆 -
克隆编号
6E9B12D5 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110262于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
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ICC/IF |
Use a concentration of 5 µg/ml.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa. |
ICC/IF
Use a concentration of 5 µg/ml. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
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功能
This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. -
序列相似性
Belongs to the cytochrome c oxidase subunit 5A family. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 444878 Cow
- Entrez Gene: 9377 Human
- Entrez Gene: 12858 Mouse
- Entrez Gene: 252934 Rat
- Omim: 603773 Human
- SwissProt: P00426 Cow
- SwissProt: P20674 Human
- SwissProt: P12787 Mouse
see all -
别名
- COX antibody
- COX VA antibody
- COX5A antibody
see all
图片
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All lanes : Anti-COX5A antibody [6E9B12D5] (ab110262) at 1 µg/ml
Lane 1 : Human heart mitochondria at 5 µg
Lane 2 : Bovine heart mitochondria at 1 µg
Lane 3 : Rat heart mitochondria at 10 µg
Lane 4 : Mouse heart mitochondria at 10 µg
Lane 5 : HepG2 mitochondria at 20 µg
Predicted band size: 17 kDa -
Mitochondrial localization of COX5A. Cultured Human embryonic lung-derived fibroblasts (strain MRC5) were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110262 (10 µg/ml) followed by an AlexaFluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody (1 µg/ml).
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HeLa cells were stained with 1 µg/mL ab110262 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
数据表及文件
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Datasheet download
文献 (51)
ab110262 被引用在 51 文献中.
- Ding Z et al. The Quality Changes and Proteomic Analysis of Cattle Muscle Postmortem during Rigor Mortis. Foods 11:N/A (2022). PubMed: 35053949
- Li K et al. Maternally Inherited Diabetes Mellitus Associated with a Novel m.15897G>A Mutation in Mitochondrial tRNAThr Gene. J Diabetes Res 2020:2057187 (2020). PubMed: 32083134
- Protasoni M et al. Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV. EMBO J 39:e102817 (2020). PubMed: 31912925
- Lobo-Jarne T et al. Multiple pathways coordinate assembly of human mitochondrial complex IV and stabilization of respiratory supercomplexes. EMBO J N/A:e103912 (2020). PubMed: 32511785
- Pajuelo Reguera D et al. Cytochrome c Oxidase Subunit 4 Isoform Exchange Results in Modulation of Oxygen Affinity. Cells 9:N/A (2020). PubMed: 32075102