Application
Western blot
Sample
Human Cell lysate - whole cell
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (Reduced Denaturing (Reduced Denaturing (4-15%))
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Concentration
1 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST 5% milk
Secondary antibody
Name
Non-Abcam antibody was used: Santa Cruz sc-2054 mouse/human adsorbed
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
Pierce West Pico
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: no kDa Non-specific: 15 kDa
Positive control
Line1: Whole cell lysate of HeLa WT cells
Negative control
Line2: Whole cell lysate of HeLa rho-0 cells. These cells do not have mitochondrial DNA
Additional data
Additional Notes
No specific ban is observed.
PVDF membrane (Pierce), lysis buffer 10 mM Tris, pH=8.0, 0.5% SDS, 1x protease inhibitor cocktail Pierce PI-87875. Protein determination: BCA (Pierce)
PVDF membrane (Pierce), lysis buffer 10 mM Tris, pH=8.0, 0.5% SDS, 1x protease inhibitor cocktail Pierce PI-87875. Protein determination: BCA (Pierce)
Abcam response
Thank you for your honest and valuable feedback. We are sorry this antibody has not worked under your experimental conditions. Many thanks for your feedback on this antibody, we hope that this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 09 2012