Application
Western blot
Sample
Human Tissue lysate - whole (human brain)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
20 µg
Treatment
huntington's disease
Specification
human brain
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% nonfat milk in TBS + 0.1% tween
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Amersham ECL prime
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 75 kDa Non-specific: 48, 120, 150 kDa
Positive control
Cox2 recombinant (not shown)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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提交于 May 16 2017