Application
Western blot
Sample
Human Tissue lysate - whole (Abdominal endometriotic lesion)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
15 µg
Specification
Abdominal endometriotic lesion
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Non fat skim milk in PBST (0.05%)
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: GE NA934-1ML Rabbit IG, HRP-linked Whole Ab, Donke
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Clarity Max Western ECL
Negative control
Water - Lane 4
Exposure
30 second(s)
Bands
Specific: 60 kDa
Positive control
Abdominal endometriotic lesion
Additional data
Additional Notes
Protein extracted using RIPA buffer (Thermo Scientific) with protease and phosphatase inhibitor cocktail (Thermo Scientific). Blot is low fluorescence PVDF membrane (BioRad). Lane 1=15 ug, Lane 2 = 7.5 ug, Lane 3 = 3.75 ug, Lane 4 = 0 ug
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 21 2017