重组
RabMAb

Anti-COX1 / Cyclooxygenase 1抗体[EPR5866] (ab109025)

概述

  • 产品名称
    Anti-COX1 / Cyclooxygenase 1抗体[EPR5866]
    参阅全部 COX1 / Cyclooxygenase 1 一抗
  • 描述
    兔单克隆抗体[EPR5866] to COX1 / Cyclooxygenase 1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IP, IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human COX1/ Cyclooxygenase 1 aa 100-200. The exact sequence is proprietary.

  • 阳性对照
    • NIH3T3, HACAT, Neuro -2a, C2C12, A431, and L6 cell lysates; Human skin tissue; HeLa cells. Mouse kidney and rat kidney tissues.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab109025 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 69 kDa.
IP 1/10 - 1/100.
IHC-P 1/150.

Heat up to 98 °C, below boiling, and then let cool for 10-20 min.

For unpurified use at 1/250 - 1/500.

ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
  • 通路
    Lipid metabolism; prostaglandin biosynthesis.
  • 序列相似性
    Belongs to the prostaglandin G/H synthase family.
    Contains 1 EGF-like domain.
  • 细胞定位
    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • COX 1 antibody
    • COX 3 antibody
    • COX-1 antibody
    • COX1 antibody
    • Cox3 antibody
    • Cyclooxygenase 1 antibody
    • Cyclooxygenase 3, included antibody
    • Cyclooxygenase-1 antibody
    • EC 1.14.99.1 antibody
    • Partial COX1 proteins, included antibody
    • PCOX1 antibody
    • PGG/HS antibody
    • PGH synthase 1 antibody
    • PGH1_HUMAN antibody
    • PGHS-1 antibody
    • PGHS1 antibody
    • PHS 1 antibody
    • PHS1 antibody
    • Prostaglandin G/H synthase 1 antibody
    • Prostaglandin H2 synthase 1 antibody
    • Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) antibody
    • Prostaglandin-endoperoxide synthase 1 antibody
    • PTGHS antibody
    • PTGS1 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling COX1 / Cyclooxygenase 1 with Purified ab109025 at 1:150 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
  • All lanes : Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025) at 1/10000 dilution (purified)

    Lane 1 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates
    Lane 2 : L6 (Rat skeletal muscle myoblast) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 69 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025) at 1/2000 dilution (purified)

    Lane 1 : HaCaT (Human skin keratinocyte)whole cell lysates
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 69 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • ab109025 (purified) at 1:20 dilution (0.8μg) immunoprecipitating COX1 / Cyclooxygenase 1 in C2C12 whole cell lysate.

    Lane 1 (input): C2C12 (Mouse myoblasts myoblast) whole cell lysate,10μg
    Lane 2 (+): ab109025 & C2C12 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109025 in C2C12 whole cell lysate

    For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Flow Cytometry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling COX1 / Cyclooxygenase 1 with purified ab109025 at 1:100 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor ® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling COX1 / Cyclooxygenase 1 with Purified ab109025 at 1:150 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling COX1 / Cyclooxygenase 1 with Purified ab109025 at 1:150 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
  • Immunocytochemistry/Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma) labelling COX1 with purified ab109025 at 1/50. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

  • All lanes : Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025) at 1/1000 dilution (unpurified)

    Lane 1 : NIH3T3 cell lysate
    Lane 2 : HACAT cell lysate
    Lane 3 : Neuro 2a cell lysate
    Lane 4 : C2C12 cell lysate
    Lane 5 : A431 cell lysate
    Lane 6 : L6 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 69 kDa

  • Unpurified ab109025 at 1/250 dilution staining COX1 / Cyclooxygenase 1 in Human skin by Immunohistochemistry, Paraffin-embedded tissue.

  • Unpurified ab109025 at 1/100 dilution staining COX1 / Cyclooxygenase 1 in HeLa cells by Immunofluorescence.

  • Overlay histogram showing NIH3T3 cells stained with unpurified ab109025 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109025, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

文献

This product has been referenced in:
  • Jensen TSR  et al. Combined activity of COX-1 and COX-2 is increased in non-neoplastic colonic mucosa from colorectal neoplasia patients. BMC Gastroenterol 18:31 (2018). Read more (PubMed: 29486731) »
  • Chang EY  et al. Inhibition of Prostaglandin Reductase 2, a Putative Oncogene Overexpressed in Human Pancreatic Adenocarcinoma, Induces Oxidative Stress-Mediated Cell Death Involving xCT and CTH Gene Expressions through 15-Keto-PGE2. PLoS One 11:e0147390 (2016). WB ; Human . Read more (PubMed: 26820738) »

See all 8 Publications for this product

客户评价及客户问答

Thank you for your inquiry.

I am happy toconfirm that the image on the datasheet was produced using Citrate buffer pH6 for antigen retrieval. Therefore I suggest to also use this buffer in your experiments with high temperature.

The...

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Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab109025 inIHC auf gefrorenen Schnitten (IHC-Fr) zu testen, annehmen möchten. Entschuldigen Sie bitte, dass ich es nicht mehr geschafft habe, Ihnen den Code ges...

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Thank you for contacting Abcam.



The antibody ab109025 would not recognize the protein that is missing the first 250 amino acids. The peptide maps between amino acids 120 and 145 of human Prostaglandin G/H synthase 1/COX1 (UniProt: ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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