Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T)
Total protein in input
200 µg
Specification
293T
Immuno-precipitation step
Protein G
Other product details
Concentration
4 µg/mg lysate
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: RIPA Buffer
Western Blot antibody
Name
Abcam antibody:Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4]
Concentration
1 µg/ml
Additional data
Additional Notes
This antibody immunoprecipitates the dominant species of 60 kDa observed in 293T cell extract (and numerous other cell extracts) by western blot (see abreview of 7th November 2007 for further information).
Lane order:
1, CoxIV IP 2, Con IgG: 3, Input (10 %)
Lane order:
1, CoxIV IP 2, Con IgG: 3, Input (10 %)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Dec 07 2007