Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH6
Permeabilization
No
Specification
heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/600
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Cy5 donkey anti-rabbit secondary antibody
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy5 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy5 ®
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 02 2015