Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (Jurkats)
Specification
Jurkats
Treatment
TCR activation with OKT3 and anti CD28
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: chicken anti mouse
Host species: Chicken
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Chicken
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Bands
Specific: ~15 kDa
Exposure
30 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Tejpal Gill
Verified customer
提交于 Jan 13 2014