Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (trochlea bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
trochlea bone
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 0.24% · Temperature: rt°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: pbs
Dilution
1/400
Secondary antibody
Name
Non-Abcam antibody was used: dako Real detection system AP/RED
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Additional data
Additional Notes
Dilution of primary antibody need to be better : maybe testing 1/500 ou 1/600 to reduce the background.
COMP stain cells of the cartilage.
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提交于 Jun 06 2017