Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human cartilage)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9.0, microwave
Permeabilization
Yes - 0.2% Triton X-100 and PBS
Specification
human cartilage
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: overnight in PBS
Dilution
1/10
Secondary antibody
Name
Non-Abcam antibody was used: Vectastain ABC Kit
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
IHC -Vector Laboratories DAB Substrate kit for peroxidase + nickel was used.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Steffen Rickelt
Verified customer
提交于 Mar 19 2014