Application
Western blot
Sample
Mouse Tissue lysate - whole (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Buffer
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Ig
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: none kDa Non-specific: >200, ~25 kDa
Positive control
HEK293 lysate
Additional data
Additional Notes
Goat anti-rabbit antibody was used as secondary antibody
Abcam response
Thank you for providing results of your testing in mouse. Unfortunately, it does not seem to be successful on this occasion as there is no band at expected MW. We encourage further Abtrial testing, if of interest.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jun 02 2016