Anti-CLEC4E/MINCLE抗体[AT16E3] (ab100846)
Key features and details
- Mouse monoclonal [AT16E3] to CLEC4E/MINCLE
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
概述
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产品名称
Anti-CLEC4E/MINCLE抗体[AT16E3]
参阅全部 CLEC4E/MINCLE 一抗 -
描述
小鼠单克隆抗体[AT16E3] to CLEC4E/MINCLE -
宿主
Mouse -
经测试应用
适用于: IHC-P, WB, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human CLEC4E/MINCLE aa 41-219.
Database link: NP_055173 -
阳性对照
- HeLa cells and HeLa whole cell lysate (ab150035)
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常规说明
This product was changed from ascites to tissue culture supernatant on 08/08/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
AT16E3 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab100846于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
说明 |
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IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
靶标
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功能
C-type lectin that functions as cell-surface receptor for a wide variety of ligands such as damaged cells, fungi and mycobacteria. Plays a role in the recognition of pathogenic fungi, such as Candida albicans. The detection of mycobacteria is via trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid. Specifically recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia. Recognizes also SAP130, a nuclear protein, that is released by dead or dying cells. Transduces signals through an ITAM-containing adapter protein, Fc receptor gamma chain /FCER1G. Induces secretion of inflammatory cytokines through a pathway that depends on SYK, CARD9 and NF-kappa-B. -
序列相似性
Contains 1 C-type lectin domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 26253 Human
- Omim: 609962 Human
- SwissProt: Q9ULY5 Human
- Unigene: 236516 Human
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别名
- C type lectin domain family 4 member E antibody
- C type lectin superfamily member 9 antibody
- C-type (calcium dependent carbohydrate recognition domain) lectin superfamily member 9 antibody
see all
图片
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Anti-CLEC4E/MINCLE antibody [AT16E3] (ab100846) at 1/1000 dilution + HeLa Cell Lysate at 35 µg
Secondary
goat anti-mouse conjugated to HRP
Developed using the ECL technique.
Predicted band size: 25 kDaThis image was generated using the ascites version of the product.
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Immunofluorescence of Human HeLa cells stained with ab100846 (1/500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).
This image was generated using the ascites version of the product.
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ab100846 staining CLEC4E/MINCLE in colorectal cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were subjected to antigen retrieval in 0.1M citric acid. The primary antibody was diluted 1/50 and incubated with the sample for 2 hours at room temperature. Antibody was detected by DAB staining.
This image was generated using the ascites version of the product.
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Overlay histogram showing HeLa cells stained with ab100846 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab100846, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91633, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab100846 被引用在 2 文献中.
- Zhao X et al. Activation of C-Type Lectin Receptor and (RIG)-I-Like Receptors Contributes to Proinflammatory Response in Middle East Respiratory Syndrome Coronavirus-Infected Macrophages. J Infect Dis 221:647-659 (2020). PubMed: 31562757
- Seifert L et al. The necrosome promotes pancreatic oncogenesis via CXCL1 and Mincle-induced immune suppression. Nature 532:245-9 (2016). PubMed: 27049944