Anti-CKAP1/TBCB抗体(ab96101)
Key features and details
- Rabbit polyclonal to CKAP1/TBCB
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-CKAP1/TBCB抗体
参阅全部 CKAP1/TBCB 一抗 -
描述
兔多克隆抗体to CKAP1/TBCB -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow -
免疫原
Recombinant fragment corresponding to Human CKAP1/TBCB aa 1-213.
Database link: NP_001272 -
阳性对照
- WB: A431, H1299, HeLa and NIH/3T3 whle cell lysates. IHC-P: H1299 xenograft ICC/IF: A549 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96101于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 27 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 27 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth. -
组织特异性
Found in most tissues. -
序列相似性
Belongs to the TBCB family.
Contains 1 CAP-Gly domain. -
翻译后修饰
Phosphorylation by PAK1 is required for normal function. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated in the presence of GAN which targets it for degradation by the proteasome. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton. Colocalizes with microtubules. In differentiated neurons, located in the cytoplasm. In differentiating neurons, accumulates at the growth cone. - Information by UniProt
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数据库链接
- Entrez Gene: 1155 Human
- Entrez Gene: 66411 Mouse
- Entrez Gene: 292777 Rat
- Omim: 601303 Human
- SwissProt: Q99426 Human
- SwissProt: Q9D1E6 Mouse
- Unigene: 31053 Human
- Unigene: 27947 Mouse
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别名
- CG22 antibody
- CKAPI antibody
- Cytoskeleton associated protein 1 antibody
see all
图片
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All lanes : Anti-CKAP1/TBCB antibody (ab96101) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : Hela whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 27 kDa
12% SDS PAGE -
Methanol-fixed A549 (human lung carcinoma cell line) cells labeling CKAP1/TBCB using ab96101 at 1/500 dilution (green) in ICC/IF.
The nuclear counter stain is Hoechst 33342 (blue).
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ab96101, at a 1/500 dilution, staining CKAP1/TBCB in paraffin embedded H1299 xenograft by Immunohistochemical analysis.
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Anti-CKAP1/TBCB antibody (ab96101) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 27 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab96101 被引用在 1 文献中.
- Gong J et al. Expression of tubulin folding cofactor B in mouse hepatic ischemia-reperfusion injury. Biomed Rep 6:525-531 (2017). PubMed: 28515911