Application
Western blot
Sample
Human Cell lysate - whole cell (293T cell)
Loading amount
30 µg
Specification
293T cell
Gel Running Conditions
Reduced Denaturing (7.5% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/2500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% skim-milk in PBS-T
Secondary antibody
Name
Abcam antibody:Mouse anti-Human IgG heavy chain secondary antibody
Dilution
1/10000
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 260 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Min Young Kim
Verified customer
提交于 Dec 31 2012