Anti-CHCHD10抗体(ab121196)
Key features and details
- Rabbit polyclonal to CHCHD10
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CHCHD10抗体
参阅全部 CHCHD10 一抗 -
描述
兔多克隆抗体to CHCHD10 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence, corresponding amino acids 43-142 (GLMAQMATTA AGVAVGSAVG HVMGSALTGA FSGGSSEPSQ PAVQQAPTPA APQPLQMGPC AYEIRQFLDC STTQSDLSLC EGFSEALKQC KYYHGLSSLP) of Human CHCHD10.
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阳性对照
- IHC-P: Human colon, skeletal muscle and liver tissue. ICC/IF: A-431 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121196于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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说明 |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
靶标
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序列相似性
Contains 1 CHCH domain. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 400916 Human
- SwissProt: Q8WYQ3 Human
- Unigene: 66915 Human
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别名
- C22orf16 antibody
- CHC10_HUMAN antibody
- CHCHD10 antibody
see all
图片
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Immunofluorescent staining of Human cell line A-431 shows positivity in mitochondria. Recommended concentration of ab121196 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemical analysis of human colon tissue sections labeling CHCHD10 with ab121196 at 1/200.
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Immunohistochemical analysis of human liver tissue sections labeling CHCHD10 with ab121196 at 1/200.
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Immunohistochemical analysis of human skeletal muscle tissue sections labeling CHCHD10 with ab121196 at 1/200.
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Immunohistochemical analysis of human skin tissue sections labeling CHCHD10 with ab121196 at 1/200.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab121196 被引用在 4 文献中.
- Kee TR et al. Pathological characterization of a novel mouse model expressing the PD-linked CHCHD2-T61I mutation. Hum Mol Genet 31:3987-4005 (2022). PubMed: 35786718
- Liu T et al. Modulation of synaptic plasticity, motor unit physiology, and TDP-43 pathology by CHCHD10. Acta Neuropathol Commun 10:95 (2022). PubMed: 35787294
- Liu T et al. CHCHD10-regulated OPA1-mitofilin complex mediates TDP-43-induced mitochondrial phenotypes associated with frontotemporal dementia. FASEB J 34:8493-8509 (2020). PubMed: 32369233
- Lehmer C et al. A novel CHCHD10 mutation implicates a Mia40-dependent mitochondrial import deficit in ALS. EMBO Mol Med 10:N/A (2018). WB, ICC/IF . PubMed: 29789341