Application
Western blot
Sample
Human Cell lysate - whole cell (hek293 CELLS)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
40 µg
Specification
hek293 CELLS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: RT°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: GOAT ANTI RABBIT THERMOFISHER
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ecl+
Exposure
15 second(s)
Bands
Specific: 290 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Amani Hassan
Verified customer
提交于 Mar 25 2017