Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human brain motor cortex)
Antigen retrieval step
Heat mediated
Specification
Human brain motor cortex
Blocking step
Protein Block Novocastra from Leica Biosystems (# RE7102-CE) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Formalin
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Tris
Dilution
1/500
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, Ale
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MRS. Guinevere Lourenco
Verified customer
提交于 Nov 20 2015