Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (C3H10T cells)
Specification
C3H10T cells
Treatment
25ng/ml (BMP4)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Bands
Specific: 38, 35, 20 kDa
Exposure
30 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 26 2015