Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1x Dako Target Retrival Solution in Water
Permeabilization
Yes - 0.5% Tween-20 in PBS
Specification
Colon
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Concentration
2 µg/ml
Secondary antibody
Dilution
1/800
Name
Non-Abcam antibody was used: anti-Goat IgG (H&L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
ab48009 did not stain Cdx2 in human colon. Controls for detection of Cdx2 and for the secondary antibody used were positive, verifying the ab48009 result as a "true" negative.
Image Legend: Ab48009 failed to stain CDX2 in human colon detected by immunofluorescence. Tissue (A-C human colon D mouse placenta) was fixed in formalin, embedded in paraffin and sectioned at 5´m. After antigen retrieval sections were blocked and incubated with primary antibody (A ab48009 2 ´g/ml, B control mouse mAb anti-human CDX2, C mouse and goat IgG 2 ´g/ml, D control goat anti-mouse CD31), washed and visualized with Alexa Fluor488-conjugated to donkey anti-goat (A,C,D) or Alexa Fluor594-conjugated to donkey anti-mouse (B,C) IgG polyclonal secondary antibody (1/800). DNA stained with DAPI.
Abcam response
We would like to thank the reviewer for submitting an Abreview for this product.
As the product had not yet been tested in IHC-P applications with human sample types, we were unsure what the result might be; we appreciate the time and effort that the reviewer has put in to testing this product in this application.
Further optimization may be necessary for use of this product in IHC-P with human sample types, and we are happy to help troubleshoot any products or applications which may not be performing.
We do encourage all of our customers to contact us should they wish to test any of our products in untested species or applications, as those products may qualify for our AbTrial Testing Program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 22 2017