重组Anti-CDX2抗体[EPR2764Y] - BSA and Azide free (ab220799)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2764Y] to CDX2 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-CDX2抗体[EPR2764Y] - BSA and Azide free
参阅全部 CDX2 一抗 -
描述
兔单克隆抗体[EPR2764Y] to CDX2 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt (Intra),ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Rabbit -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- IHC-P: human gastric adenocarcinoma tissue. human colonic adenocarcinoma and human, rat and mouse colon tissue.
-
常规说明
ab220799 is the carrier-free version of ab76541.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解离常数(KD)
KD = 1.00 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2764Y -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
- Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)
- Alexa Fluor® 647 Anti-CDX2 antibody [EPR2764Y] (ab195008)
- PE Anti-CDX2 antibody [EPR2764Y] (ab303008)
- APC Anti-CDX2 antibody [EPR2764Y] (ab303009)
- HRP Anti-CDX2 antibody [EPR2764Y] (ab303010)
- Alexa Fluor® 555 Anti-CDX2 antibody [EPR2764Y] (ab312266)
- Alexa Fluor® 568 Anti-CDX2 antibody [EPR2764Y] (ab312755)
- Anti-CDX2 antibody [EPR2764Y] (ab76541)
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab220799于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
|
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
说明 |
---|
WB
Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
-
功能
Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. -
序列相似性
Belongs to the Caudal homeobox family.
Contains 1 homeobox DNA-binding domain. -
翻译后修饰
Phosphorylation of Ser-60 mediates the transactivation capacity. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 1045 Human
- Entrez Gene: 12591 Mouse
- Entrez Gene: 66019 Rat
- Omim: 600297 Human
- SwissProt: Q99626 Human
- SwissProt: P43241 Mouse
- Unigene: 174249 Human
- Unigene: 20358 Mouse
-
别名
- Caudal type homeo box 2 antibody
- Caudal type homeo box transcription factor 2 antibody
- Caudal type homeobox 2 antibody
see all
图片
-
This image was made using ab76541 which is the same antibody as ab220799 with BSA and Azide
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat colon tissue sections labeling CDX2 with Purified ab76541 at 1:1000 dilution (0.8 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051
was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
This image was made using ab76541 which is the same antibody as ab220799 with BSA and Azide
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue sections labeling CDX2 with Purified ab76541 at 1:1000 dilution (0.8 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051
was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain. -
This image was made using ab76541 which is the same antibody as ab220799 with BSA and Azide
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling CDX2 with Purified ab76541 at 1:1000 dilution (0.8 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051
was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain. -
Unpurified ab76541, at 1/250 dilution, staining CDX2 in paraffin-embedded human gastric adenocarcinoma tissue, by immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76541).
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
-
Unpurified ab76541 staining CDX2 in Human intestinal tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 1 hours at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in PBS containing 5% BSA) for 18 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76541).
-
Unpurified ab76541 showing positive staining in human Colonic adenocarcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76541).
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
-
Unpurified ab76541 showing positive staining in Normal human colon tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76541).
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
-
Unpurified ab76541 showing negative staining in human Skeletal muscle tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76541).
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
-
Unpurified ab76541 showing negative staining in Normal human kidney tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76541).
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
-
Unpurified ab76541 showing negative staining in Normal human brain tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76541).
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
-
Unpurified ab76541 showing negative staining in Normal human brain tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76541).
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
-
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76541).
实验方案
数据表及文件
-
Datasheet download
Certificate of Compliance
文献 (11)
ab220799 被引用在 11 文献中.
- Yang Y et al. Heightened potency of human pluripotent stem cell lines created by transient BMP4 exposure. Proc Natl Acad Sci U S A 112:E2337-46 (2015). PubMed: 25870291
- Ashley N et al. Rapidly derived colorectal cancer cultures recapitulate parental cancer characteristics and enable personalized therapeutic assays. J Pathol 234:34-45 (2014). Human . PubMed: 24797403
- Ohinata Y & Tsukiyama T Establishment of trophoblast stem cells under defined culture conditions in mice. PLoS One 9:e107308 (2014). ICC/IF . PubMed: 25203285
- Amita M et al. Complete and unidirectional conversion of human embryonic stem cells to trophoblast by BMP4. Proc Natl Acad Sci U S A 110:E1212-21 (2013). ICC/IF ; Human . PubMed: 23493551
- Sakaki-Yumoto M et al. Smad2 is essential for maintenance of the human and mouse primed pluripotent stem cell state. J Biol Chem 288:18546-60 (2013). PubMed: 23649632