Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa S3 cervical cancer)
Gel Running Conditions
Reduced Denaturing (8% Tris-glycine SDS-PAGE)
Loading amount
50 µg
Treatment
double thymidine block and release
Specification
HeLa S3 cervical cancer
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-Tween w/5% milk
Dilution
1/1000
Detection
Detection method
ECL Prime
Negative control
Time point corresponding to late S phase had reduced Cdt1 as predicted.
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 63 kDa
Positive control
G1 timepoint, corresponding to 10 hrs post release showed high levels of Cdt1.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 05 2012