Application
Western blot
Sample
Mouse Cell lysate - whole cell (Balb MK/ mouse keratinocyte / and 3T3 / and CHO)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
500000 cells
Specification
Balb MK/ mouse keratinocyte / and 3T3 / and CHO
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS-T, 3% milk
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: jackson antirabbit HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: see no band for mous kDa Non-specific: 52 KDa for mouse, CHO kDa
Positive control
HeLa total cell lysate was used. A band about 63kDa was observed in Hela
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Thinh Cao
Verified customer
提交于 May 07 2009