Application
Western blot
Sample
Mouse Tissue lysate - whole (Inguinal Adipose Tissue)
Gel Running Conditions
Reduced Denaturing (2-20% Precast Gel, 100V for 1 hr 20 min to run)
Loading amount
40 µg
Specification
Inguinal Adipose Tissue
Blocking step
SuperBlock T20 (TBS) Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Overnight
Dilution
1/500
Detection
Detection method
ECL
Negative control
Tubulin at 50kDa
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: None kDa Non-specific: 70 kDa
Additional data
Additional Notes
Lane 1 = Ladder
Lane 2 = iWAT 1
Lane 3 = iWAT 2
Lane 4 = iWAT 3
Lane 5 = iWAT 4
Lane 6 = Ladder
Lane 7 = iWAT 1
Lane 8 = iWAT 2
Lane 9 = iWAT 3
Lane 10 = iWAT 4
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Sep 13 2016