Western blot abreview for Anti-CDKN2A/p16INK4a antibody [2D9A12]

Average
Abreviews
Application Western blot
Sample Mouse Tissue lysate - whole (Inguinal Adipose Tissue)
Gel Running Conditions Reduced Denaturing (2-20% Precast Gel, 100V for 1 hr 20 min to run)
Loading amount 40 µg
Specification Inguinal Adipose Tissue
Blocking step SuperBlock T20 (TBS) Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C

Other product details

Incubation time 16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Overnight
Dilution 1/500

Secondary antibody

Dilution 1/2000
Name Abcam antibody:Goat Anti-Mouse IgG H&L (HRP)

Detection

Detection method ECL
Negative control Tubulin at 50kDa
Exposure 2 minute(s) and 0 second(s)
Bands Specific: None kDa Non-specific: 70 kDa

Additional data

Additional Notes Lane 1 = Ladder Lane 2 = iWAT 1 Lane 3 = iWAT 2 Lane 4 = iWAT 3 Lane 5 = iWAT 4 Lane 6 = Ladder Lane 7 = iWAT 1 Lane 8 = iWAT 2 Lane 9 = iWAT 3 Lane 10 = iWAT 4
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提交于 Sep 13 2016