Western blot abreview for Anti-CDKN2A/p16INK4a antibody [2D9A12]

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (15%gel)
Sample Mouse Tissue lysate - whole (hippocampus)
Specification hippocampus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time 12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution 1/500

Secondary antibody

Name Non-Abcam antibody was used: anti-mouse santacruz
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/1000


Detection method ECL
Bands Specific: 20 kDa
Exposure 10 minute(s) and 0 second(s)

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提交于 Apr 10 2015