Western blot abreview for Anti-CDKN2A/p16INK4a antibody [2D9A12]

Good
Abreviews
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (15%gel)
Sample
Mouse Tissue lysate - whole (hippocampus)
Specification
hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: anti-mouse santacruz
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
ECL
Bands
Specific: 20 kDa
Exposure
10 minute(s) and 0 second(s)
Username

Abcam user community

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提交于 Apr 10 2015

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