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I’m wondering if you might be able to help with the specificity of your WB antibodies for Ink4a/p16? Our model is the Syrian hamster (Mesocricetus auratus); I know that previously we had tried ab54210 (human and rat) but were getting multiple bands due to the overlapping transcripts at that locus (p16, p15 and ARF) – There are several Ink4a antibodies listed on your catalogue but I’m not sure which is specific for p16 only? Alternatively and possibly more useful to me; is there accessible information as to which amino acids /epitope the antibody binds to so as to be able to predict the specificity from protein alignments?
Asked on Aug 30 2012
Thank you for your enquiry and your interest in our products.
We have several antibodies against CDKN2A but none of them have been tested for cross-reactivity with Syrian hamster (Mesocricetus auratus).
Regarding ab54210 - the exact immunogen sequence of this antibody is unknown. We expressed the antigen via an expression vector provided to us so we do not have further information about the exact sequence. We have not done any related testing, so we unfortunately cannot comment on cross-reactivity with Syrian hamster. We can only guarantee reactivity with human and rat as presented on our data sheet.
My apologies, we could not provide a more definitive answer.
If you need any further assistance in the future, please do not hesitate to contact me.
Abcam Scientific Support
回复于 Aug 30 2012