Anti-CDKL5抗体(ab22453)
Key features and details
- Rabbit polyclonal to CDKL5
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-CDKL5抗体
参阅全部 CDKL5 一抗 -
描述
兔多克隆抗体to CDKL5 -
宿主
Rabbit -
特异性
Swissprot predicts mouse CDKL5 protein to be ~105 kDa (Q3UTQ8), however a recent paper by Lin et al, 2006 (PMID: 16330482) indicated CDKL5 to run at ~118 kDa on WB. On this basis we think that the ~120 kDa band we observe using Rabbit polyclonal to CDKL5 (ab22453) on mouse tissue is CDKL5 specific. Futhermore the ~120 kDa band is blocked by the immunising peptide. The larger ~200 kDa is potentially also CDKL5 as a similar band is also observed in the Lin et al (2006) publication. There is evidence for larger forms of CDKL5 for the human version of the protein, so it may not be surprising to find a similar scenario for the mouse protein. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse
预测可用于: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab22453于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 120,160 kDa (predicted molecular weight: 105 kDa).
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说明 |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 120,160 kDa (predicted molecular weight: 105 kDa). |
靶标
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功能
Mediates phosphorylation of MECP2. -
组织特异性
Expressed in brain, lung, kidney, prostate, ovary, placenta, pancreas and testis. -
疾病相关
Note=Chromosomal aberrations involving CDKL5 are found in patients manifesting early-onset seizures and spams and psychomotor impairment. Translocation t(X;6)(p22.3;q14); translocation t(X;7)(p22.3;p15).
Defects in CDKL5 are a cause of epileptic encephalopathy early infantile type 2 (EIEE2) [MIM:300672]; also known as atypical CDKL5-related Rett syndrome. EIEE2 is a severe form of epilepsy characterized by seizures or spasms beginning in infancy. Patients manifest features resembling Rett syndrome such as microcephaly, lack of speech development, stereotypic hand movements. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 6792 Human
- Entrez Gene: 382253 Mouse
- Omim: 300203 Human
- SwissProt: O76039 Human
- SwissProt: Q3UTQ8 Mouse
- Unigene: 659851 Human
- Unigene: 443717 Mouse
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别名
- Cdkl5 antibody
- CDKL5_HUMAN antibody
- Cyclin dependent kinase 5 transcript antibody
see all
图片
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All lanes : Anti-CDKL5 antibody (ab22453) at 3 µg/ml
Lane 1 : mouse cortex
Lane 2 : mouse cortex with Human CDKL5 peptide (ab25756) at 2 µg/ml
Lysates/proteins at 20 µg per lane.
Predicted band size: 105 kDa
Observed band size: 120,200 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
Swissprot predicts mouse CDKL5 protein to be ~105kDa (Q3UTQ8), however a recent paper by Lin et al, 2006 (PMID: 16330482) indicated CDKL5 to run at ~118kDa on WB. On this basis we think that the ~120kDa band we observe using Rabbit polyclonal to CDKL5 (ab22453) on mouse tissue is CDKL5 specific. Futhermore the ~120kDa band is blocked by the immunising peptide. The larger >150kDa is potentially also CDKL5 as a similar band is also observed in the Lin et al (2006) publication. There is evidence for larger forms of CDKL5 for the human version of the protein, so it may not be surprising to find a similar scenario for the mouse protein.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab22453 被引用在 5 文献中.
- Clemm von Hohenberg K et al. Cyclin B/CDK1 and Cyclin A/CDK2 phosphorylate DENR to promote mitotic protein translation and faithful cell division. Nat Commun 13:668 (2022). PubMed: 35115540
- Kim JY et al. A kinome-wide screen identifies a CDKL5-SOX9 regulatory axis in epithelial cell death and kidney injury. Nat Commun 11:1924 (2020). PubMed: 32317630
- Baltussen LL et al. Chemical genetic identification of CDKL5 substrates reveals its role in neuronal microtubule dynamics. EMBO J 37:N/A (2018). PubMed: 30266824
- Wang IT et al. Loss of CDKL5 disrupts kinome profile and event-related potentials leading to autistic-like phenotypes in mice. Proc Natl Acad Sci U S A : (2012). WB . PubMed: 23236174
- Nemos C et al. Mutational spectrum of CDKL5 in early-onset encephalopathies: a study of a large collection of French patients and review of the literature. Clin Genet 76:357-71 (2009). WB, ICC/IF ; Human . PubMed: 19793311