Anti-CDK1 (phospho Y15)抗体(ab47594)
Key features and details
- Rabbit polyclonal to CDK1 (phospho Y15)
- Suitable for: WB
- Reacts with: Human, African green monkey
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CDK1 (phospho Y15)抗体
参阅全部 CDK1 一抗 -
描述
兔多克隆抗体to CDK1 (phospho Y15) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human, African green monkey -
免疫原
Synthetic peptide corresponding to Human CDK1 aa 1-100 (phospho Y15).
Database link: P06493 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47594于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa). |
靶标
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功能
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. -
组织特异性
Isoform 2 is found in breast cancer tissues. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 983 Human
- Omim: 116940 Human
- SwissProt: P06493 Human
- Unigene: 334562 Human
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形式
CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage. -
别名
- Cdc 2 antibody
- Cdc2 antibody
- CDC28A antibody
see all
图片
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Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/500 dilution + COS-7 cells (African green monkey kidney fibroblast-like)
Predicted band size: 34 kDa -
Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/500 dilution + HepG2 cell extract
Predicted band size: 34 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted? -
All lanes : Anti-CDK1 (phospho Y15) antibody (ab47594) at 1/1000 dilution
Lane 1 : untreated HeLa cell lysate
Lane 2 : HeLa cell lysate treated with nocodazole (500ng/mL) for 16 h
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : polyclonal goat HRP conjugated at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 1 minuteBlocking: 5% milk for 1 hour at 25°C.
数据表及文件
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SDS download
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Datasheet download
文献 (38)
ab47594 被引用在 38 文献中.
- Liu JCY et al. Mechanism and function of DNA replication-independent DNA-protein crosslink repair via the SUMO-RNF4 pathway. EMBO J 40:e107413 (2021). PubMed: 34346517
- Zhang X et al. Treatment of Melanoma by Nano-conjugate-Delivered Wee1 siRNA. Mol Pharm 18:3387-3400 (2021). PubMed: 34375118
- Liu J et al. Knockdown of MAPK14 inhibits the proliferation and migration of clear cell renal cell carcinoma by downregulating the expression of CDC25B. Cancer Med 9:1183-1195 (2020). PubMed: 31856414
- Gray S et al. Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner. Cell Rep 32:107858 (2020). PubMed: 32640224
- Guo X et al. RNA demethylase ALKBH5 prevents pancreatic cancer progression by posttranscriptional activation of PER1 in an m6A-YTHDF2-dependent manner. Mol Cancer 19:91 (2020). PubMed: 32429928