Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.05% Triton X 100 in PBS for 5 min
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: in blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: Molecular Probes
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
This antibody is not great for IF staining, but works well by western. Cells are asynchronous.
Abcam response
Thank you for your valuable feedback. Immunofluorescence is not a tested application for ab47329 and seems that this antibody does not recognize phospho-T161 Cdc2 under these experimental conditions. Thank you for your time - we hope that this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 06 2010