重组Anti-CDK1 + Cdk2 + Cdk3 (phospho T14)抗体[E161] (ab32384)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E161] to CDK1 + Cdk2 + Cdk3 (phospho T14)
- Suitable for: WB, IHC-P, IP, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CDK1 + Cdk2 + Cdk3 (phospho T14)抗体[E161] -
描述
兔单克隆抗体[E161] to CDK1 + Cdk2 + Cdk3 (phospho T14) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IP, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow -
免疫原
Synthetic peptide within Human CDK1 aa 1-100. The exact sequence is proprietary.
Database link: P06493 -
阳性对照
- WB: HeLa cell lysate. IHC-P: Human lymphoma.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E161 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32384于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
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IHC-P |
1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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IP |
1/50.
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ICC/IF |
1/100 - 1/500.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
IHC-P
1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
IP
1/50. |
ICC/IF
1/100 - 1/500. |
靶标
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细胞定位
CDK1: Nucleus. -
数据库链接
- Entrez Gene: 1017 Human
- Entrez Gene: 1018 Human
- Entrez Gene: 983 Human
- Entrez Gene: 12534 Mouse
- Entrez Gene: 12566 Mouse
- Entrez Gene: 362817 Rat
- Entrez Gene: 54237 Rat
- Omim: 116940 Human
see all -
形式
CDK1: CDK1 can be located to the Nucleus, cytoplasm and Mithocondria. It's cytoplasmic during interphase and reversibly translocated from cytoplasm to the nucleus when phosphorilated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
图片
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All lanes : Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (ab32384) at 1/2000 dilution (purified)
Lane 1 : Empty vector transfected 293T (human embryonic kidney) whole cell lysates
Lane 2 : CDK1-GFP transfected 293T (human embryonic kidney) whole cell lysates
Lane 3 : CDK2-GFP transfected 293T (human embryonic kidney) whole cell lysates
Lane 4 : CDK3-GFP transfected 293T (human embryonic kidney) whole cell lysates
Lane 5 : CDK5-GFP transfected 293T (human embryonic kidney) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 62, 34 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Diluting buffer 5% NFDM/TBST
34kDa band represents endogenous CDK, and 62kDa represents CDK-GFP.
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Immunohistochemical staining of paraffin embedded human B cell lymphoma with purified ab32384 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunofluorescence staining of HeLa cells with purified ab32384 at a working dilution of 1/200, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab32384 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
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All lanes : Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (ab32384) at 1/2000 dilution (purified)
Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysates
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 3mM hydroxyurea for 20 hours whole cell lysates
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 3mM hydroxyurea for 20 hours whole cell lysates. Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 30 secondsBlocking/Diluting buffer
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Immunohistochemical staining of paraffin embedded human ovarian carcinoma with purified ab32384 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (ab32384) at 1/5000 dilution (purified) + Raw264.7 cell lysate at 10 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
All lanes : Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (ab32384) at 1/10000 dilution (purified)
Lane 1 : C6 cell lysate
Lane 2 : PC-12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
ab32384 (purified) at 1/50 immunoprecipitating CDK1 in 10 μg HEK293 (Lanes 1 and 2, observed at 34 kDa). Lane 3 - PBS. For western blotting, HRP Veriblot for IP Detection Reagent (ab131366) was used for detection (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
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Unpurified ab32384, at a 1/50 dilution, staining Cdc2 in paraffin embedded human lymphoma tissue sections by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-CDK1 + Cdk2 + Cdk3 (phospho T14) antibody [E161] (ab32384) at 1/1000 dilution (unpurified)
Lane 1 : A431 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : SKBR3 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 34 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (30)
ab32384 被引用在 30 文献中.
- Wang J et al. The Circular RNA CircCOL1A1 Functions as a miR-149-5p Sponge to Regulate the Formation of Superior-Quality Brush Hair via the CMTM3/AR Axis. Front Cell Dev Biol 10:760466 (2022). PubMed: 35186916
- Beck AC et al. AP-2α Regulates S-Phase and Is a Marker for Sensitivity to PI3K Inhibitor Buparlisib in Colon Cancer. Mol Cancer Res 19:1156-1167 (2021). PubMed: 33753551
- Hu L et al. A novel M phase blocker, DCZ3301 enhances the sensitivity of bortezomib in resistant multiple myeloma through DNA damage and mitotic catastrophe. J Exp Clin Cancer Res 39:105 (2020). PubMed: 32517809
- Baptista Moreno Martin AC et al. [10]-Gingerol improves doxorubicin anticancer activity and decreases its side effects in triple negative breast cancer models. Cell Oncol (Dordr) 43:915-929 (2020). PubMed: 32761561
- Blasco N et al. Involvement of the mitochondrial nuclease EndoG in the regulation of cell proliferation through the control of reactive oxygen species. Redox Biol 37:101736 (2020). PubMed: 33032073