Application
Western blot
Sample
Mouse Cell lysate - whole cell (MPRO (mouse promyelocytic) cells)
Loading amount
50 µg
Specification
MPRO (mouse promyelocytic) cells
Gel Running Conditions
Reduced Denaturing (12% Bis-TRIS in MOPS buffer)
Blocking step
Odyssey Blocking Agent (Lycor system) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk/TBST
Secondary antibody
Name
Non-Abcam antibody was used: Lycor Goat anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/10000
Detection
Detection method
Lycor Scanner
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 21 kDa
Additional data
Additional Notes
Legend
Lane 1:HEK293 human cell lysate
Lane 2: MPRO mouse cell lysate
Lane 3: S. Cerevisiae lysate
Cells were lysed under native conditions by incubation on ice for 20 minutes in lysis buffer (50mM Tris pH 7.9, 150mM NaCl, 0.5% NP40, 1 mM EDTA, 5% glycerol, with 1 mM DTT and protease inhibitors), followed by a 30 minute cold spin to pellet debris.
Lane 1:HEK293 human cell lysate
Lane 2: MPRO mouse cell lysate
Lane 3: S. Cerevisiae lysate
Cells were lysed under native conditions by incubation on ice for 20 minutes in lysis buffer (50mM Tris pH 7.9, 150mM NaCl, 0.5% NP40, 1 mM EDTA, 5% glycerol, with 1 mM DTT and protease inhibitors), followed by a 30 minute cold spin to pellet debris.
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提交于 Feb 18 2010