Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton x-100 in PBS
Blocking step
Milk as blocking agent for 40 minute(s) · Concentration: 10% · Temperature: 23°C
Other product details
Dilution
1/140
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: 1% non-fat dry milk in PBS
Secondary antibody
Name
Non-Abcam antibody was used: AlexaFluor488 goat anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/250
Additional data
Additional Notes
Nice networks at cell edges were seen in some cases, however, non-specific binding and staining of the nucleai was often observed.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MRS. Merly Saare
Verified customer
提交于 Sep 14 2009