Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa, Jurkat)
Loading amount
500 µg
Specification
HeLa, Jurkat
Gel Running Conditions
Reduced Denaturing (12,5% acrylamide gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Other product details
Dilution
1/250
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% non-fat dry milk in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Alkaline-phosohatase anti-Rabbit IgG (H+L) (Vector
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Dilution
1/300
Detection
Detection method
Alkaline Phosphatase reaction
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 21 kDa Non-specific: 100, 72 kDa
Additional data
Additional Notes
Very weak signal even at high concentrations (datasheet recommended using 1/500 dilution, this test was done with 1/250 dilution).
Abcam response
Thank you for this valuable feedback.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MRS. Merly Saare
Verified customer
提交于 Dec 07 2009