Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,1% Triton-X-100 in PBS
Blocking step
0,2 % Gelatine in PBS as blocking agent for 10 minute(s) · Concentration: 2000µg/mL · Temperature: 22°C
Other product details
Dilution
1/50
Incubation time
40 minute(s) · Temperature: 22°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse Alexa568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Dilution
1/50
Additional data
Additional Notes
Picture: HeLa cells were stained with antiCD98 (1:50) and goat-anti-mouse-Alexa568 (1:50). The cells were PFA fixed (3%, 20 min) and permeabilized in 0,1 %Trition-X-100 (5 min). The duration of antibody-incubation was 40 min. (microscope: Zeiss LSM)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Dagmar Salber
Verified customer
提交于 Feb 11 2008