重组Anti-CD90 / Thy1抗体[EPR3132] - Low endotoxin,Azide free (ab221607)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3132] to CD90 / Thy1 - Low endotoxin, Azide free
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD90 / Thy1抗体[EPR3132] - Low endotoxin,Azide free
参阅全部 CD90 / Thy1 一抗 -
描述
兔单克隆抗体[EPR3132] to CD90 / Thy1 - Low endotoxin,Azide free -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Wild-type U-2 OS cell lysate, Human fetal brain and fetal kidney lysate. IHC-P: Human lung adenocarcinoma, pancreatic carcinoma, cervical carcinoma, breast carcinoma, colon carcinoma, glioma, tonsil and brain tissues, human skeletal muscle vessels tissue and normal human tonsil vessels tissues.
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常规说明
ab221607 is the carrier-free version of ab92574.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解离常数(KD)
KD = 1.57 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3132 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-CD90 / Thy1 antibody [EPR3132] - BSA and Azide free (ab181885)
- Alexa Fluor® 555 Anti-CD90 / Thy1 antibody [EPR3132] (ab307267)
- Alexa Fluor® 647 Anti-CD90 / Thy1 antibody [EPR3132] (ab311183)
- Alexa Fluor® 594 Anti-CD90 / Thy1 antibody [EPR3132] (ab311841)
- Alexa Fluor® 568 Anti-CD90 / Thy1 antibody [EPR3132] (ab313124)
- Anti-CD90 / Thy1 antibody [EPR3132] (ab92574)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab221607于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
The use of an HRP/AP polymerized antibody is recommended for a secondary antibody. |
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WB |
Use at an assay dependent concentration.
Observed molecular weight may vary depending on the glycosylation level of the target. |
说明 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody. |
WB
Use at an assay dependent concentration. Observed molecular weight may vary depending on the glycosylation level of the target. |
靶标
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功能
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7070 Human
- Entrez Gene: 24832 Rat
- Omim: 188230 Human
- SwissProt: P04216 Human
- SwissProt: P01830 Rat
- Unigene: 644697 Human
- Unigene: 108198 Rat
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别名
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
see all
图片
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All lanes : Anti-CD90 / Thy1 antibody [EPR3132] (ab92574) at 1/1000 dilution
Lane 1 : Wild-type U-2 OS cell lysate
Lane 2 : THY1 knockout U-2 OS cell lysate
Lane 3 : Human brain cell lysate
Lane 4 : Human kidney cell lysate
Lane 5 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Observed band size: 25-37 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
Anti-THY1 antibody [EPR3132] (ab92574) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab92574 was shown to bind specifically to THY1. A band was observed at 25-37 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in THY1 knockout cell line ab262490 (knockout cell lysate ab263925). To generate this image, wild-type and THY1 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labelling CD90 / Thy1 with ab92574 at 1/400 dilution (0.26 μg/ml) followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody at a ready to use concentration. Positive staining on the stroma in human colon carcinoma. The section was incubated with ab92574 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD90 / Thy1 with ab92574 at 1/400 dilution (0.26 μg/ml) followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody at a ready to use concentration. Positive staining on human tonsil. The section was incubated with ab92574 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.#
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
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All lanes : Anti-CD90 / Thy1 antibody [EPR3132] (ab92574) at 1/500 dilution
Lane 1 : Wild-type U-2 OS cell lysate
Lane 2 : THY1 knockout U-2 OS cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Observed band size: 35-45 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-CD90 / Thy1 antibody [EPR3132] staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab92574 was shown to bind specifically to CD90 / Thy1. A band was observed at 35-45 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in Thy1 knockout cell line ab262490 (knockout cell lysate ab263925). To generate this image, wild-type and Thy1 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
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ab92574 at 1/100 dilution staining CD90 / Thy1 in (1) Human breast carcinoma, (2) Human glioma & (3) Human brain tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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All lanes : Anti-CD90 / Thy1 antibody [EPR3132] (ab92574) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilutionThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
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ab92574 showing positive staining in Normal human tonsil vessels tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab92574 showing positive staining in human Skeletal muscle vessels tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab92574 showing positive staining in human Cervical carcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab92574 showing positive staining in human Lung adenocarcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
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Immunohistochemical analysis of paraffin-embedded Human pancreatic carcinoma tissue labeling CD90/Thy1 with ab221607 at 1/4000 dilution (0.26 μg/ml), followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on stromal cells in human pancreatic carcinoma. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
数据表及文件
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Datasheet download
Certificate of Compliance
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