Application
Western blot
Sample
Mouse Tissue lysate - whole (Ms Brain Ms Lung PC12 9L)
Loading amount
20 µg
Specification
Ms Brain Ms Lung PC12 9L
Gel Running Conditions
Reduced Denaturing (Pierce precise protein gel 8-16%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Concentration
1 µg/ml
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2.5% Non-fat milk, PBS, Tween-20 0.1%
Secondary antibody
Name
Non-Abcam antibody was used: Swine anti-Rabbit HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
Supersignal West Dura
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: 30, 60 kDa
Additional data
Additional Notes
Lane order (1-4): Ms Brain Ms Lung PC12 9L
A very strong signal was observed in Ms lung lysate at ~60 and ~30 kDa which is presumed to represent glycosylated and non-glycosylated forms of CD82. In support of this, early literature characterising CD82 (known as C33 antigen) report SDS-PAGE mobilities ranging from 30 kDa to 60 kDa representing the non-modified and glycosylated forms. See PMID: 1738199 and PMID: 1401919.
A very strong signal was observed in Ms lung lysate at ~60 and ~30 kDa which is presumed to represent glycosylated and non-glycosylated forms of CD82. In support of this, early literature characterising CD82 (known as C33 antigen) report SDS-PAGE mobilities ranging from 30 kDa to 60 kDa representing the non-modified and glycosylated forms. See PMID: 1738199 and PMID: 1401919.
Abcam response
Thank you for your honest and valuable feedback. Abcam has not tested ab66400 in rat for WB and your data suggest it may not be suitable for this species under the conditions in which you tested. Thank you for your time - we hope that this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Antibody Solutions
Verified customer
提交于 Jun 15 2010