Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Human Serum (Purified exosomes from human serum)
Specification
Purified exosomes from human serum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 4% BlockAce reagent
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit IgG, HRP-Linked Whole Ab Donkey
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: 25-26 kDa
Exposure
1 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jun 20 2014