Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cell)
Specification
mouse embryonic stem cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk (blocking buffer)
Dilution
1/1000
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Positive control
Mouse embryonic stem cells in lanes 1 and 2.
Additional data
Additional Notes
I repeated this WB using 5% BSA as a block and saw no bands. I have also tried using the antibody at a 1/100 dilution.
Abcam response
Thank you for sharing your data with us. ab109201 has previously been tested by western blot on mouse RAW264.7 cells (please see front of datasheet). However, your data shows that this antibody does not work in western blot on mouse ES cells, under these conditions. Abcam would welcome feedback from other customers using this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jan 21 2014