Application
Flow Cytometry
Sample
Human Cell (Activated CD19+ B-cells)
Specification
Activated CD19+ B-cells
Preparation
Cell harvesting/tissue preparation method: EBV transformed / activated B-cells cultured for 3 days in RPMI 1640, 10 % FCS
Sample buffer: FACS buffer (PBS, 0.5% FCS, 0.5M EDTA)
Sample buffer: FACS buffer (PBS, 0.5% FCS, 0.5M EDTA)
Fixation
None
Permeabilization
No
Gating Strategy
CD19+ B-cells
Other product details
Incubation time
20 minute(s) · Temperature: 4°C · Diluent: MACS buffer
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
This appears to be the only anti-Human CD80 APC conjugated antibody that works reliably for FACS on the market and a good product. Antibody comes in a very diluted form, so detection of low level cell surface expression of CD80 (for example on immature monocytes) is difficult, but not impossible.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Apr 27 2009