Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-20% gradient)
Sample
Dog Tissue lysate - whole (Spleen)
Specification
Spleen
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Concentration
2 µg/ml
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS, 0.1% Tween with 5% Milk
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-rabbit IgG-HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: 26 kDa
Exposure
30 second(s)
Positive control
None used antibody not yet shown to be compatible with western blotting. Sample was Canine Spleen, which is known to harbor CD8 cells.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 26 2014