Application
Immunohistochemistry (Resin sections)
Sample
Human Tissue sections (Nasal polyp and tonsil)
Specification
Nasal polyp and tonsil
Other product details
Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: Tris Buffered Saline
Secondary antibody
Name
Non-Abcam antibody was used: Biotinylated Rabbit anti Mouse (Fab2)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/1000
Additional data
Additional Notes
The tissue was embedded into GMA resin as per the Southampton HRU protocol.
The primary antibody is incubated overnight at room temperature. We find it doesn't make any difference whether this is from midday one day to 9am the next or 4pm to 9am - both will give the same results.
We currently use a CD8 antibody from a different source, which was used in the same staining run as a positive control and it gave positive staining on the same tissue.
We tried the Abcam CD8 at dilutions of 1:25 up to 1:1000.
1:500 gives the best staining - clear positive staining on the CD8+ cells with a nice clean background. It is comparable to the antibody that we have used from another supplier for over 10 years, and possibly gives an improved picture.
The primary antibody is incubated overnight at room temperature. We find it doesn't make any difference whether this is from midday one day to 9am the next or 4pm to 9am - both will give the same results.
We currently use a CD8 antibody from a different source, which was used in the same staining run as a positive control and it gave positive staining on the same tissue.
We tried the Abcam CD8 at dilutions of 1:25 up to 1:1000.
1:500 gives the best staining - clear positive staining on the CD8+ cells with a nice clean background. It is comparable to the antibody that we have used from another supplier for over 10 years, and possibly gives an improved picture.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 24 2010