Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Specification
Tonsil
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
(agent) for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Other product details
Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
small population of cells ( ?Plasma cells) are very well stained.
However, the majority of B-cells are rather pale. Increasing the concentration of the primary Ab only increases non-specific binding such that T-cells appear to be immunostained
However, the majority of B-cells are rather pale. Increasing the concentration of the primary Ab only increases non-specific binding such that T-cells appear to be immunostained
Abcam response
Thank you for submitting an Abreview. IHC-P was not a tested application for ab88525 and apparently it does not seem to work well in this experimental procedure, under these conditions. Thank you for your time, this information will surely be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Jun 02 2011