Application
Flow Cytometry
Sample
Human Cell (from buffy coat)
Specification
from buffy coat
Preparation
Cell harvesting/tissue preparation method: after ficoll purification and anti-CD3/CD28 overnight stimulation
Sample buffer: PBS/BSA 1%
Sample buffer: PBS/BSA 1%
Permeabilization
No
Gating Strategy
alive cells
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS/BSA 1%
Secondary antibody
Secondary antibody
None used
Additional data
Abcam response
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Abcam user community
Verified customer
提交于 Jan 02 2013