Application
Flow Cytometry
Fixation
no fixation
Permeabilization
No
Sample
Human Cell (basophile granulocytes)
Specification
basophile granulocytes
Gating Strategy
leucocyte gate
Preparation
Cell harvesting/tissue preparation method: peripheral blood cells, separated by Ficoll density separation, followed by magnetic isolation
Sample buffer: PBS with human IgG (Fc-block)
Sample buffer: PBS with human IgG (Fc-block)
Other product details
Concentration
8 µg/ml
Incubation time
30 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS with human IgG (Fc-block)
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
basophile granulocytes:
1 = isotype control
2 = untreated
3 = treated with anti-IgE
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 15 2013