FITC荧光Anti-CD59抗体[MEM-43],prediluted (ab18237)
Key features and details
- FITC Mouse monoclonal [MEM-43] to CD59, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Related conjugates and formulations
概述
-
产品名称
FITC荧光Anti-CD59抗体[MEM-43],prediluted
参阅全部 CD59 一抗 -
描述
FITC荧光小鼠单克隆抗体[MEM-43] to CD59,prediluted -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
特异性
The antibody reacts with a well defined epitope (W40, R-53) on CD59 molecule. -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD59. Human thymocytes and T lymphocytes
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: PBS, BSA -
Concentration information loading...
-
克隆
单克隆 -
克隆编号
MEM-43 -
同种型
IgG2a -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab18237于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use 20µl for 106 cells.
20 µl for a 100 µl sample is recommended.
ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
说明 |
---|
Flow Cyt
Use 20µl for 106 cells. 20 µl for a 100 µl sample is recommended.
ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
靶标
-
功能
Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. -
疾病相关
Defects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300]. -
序列相似性
Contains 1 UPAR/Ly6 domain. -
翻译后修饰
N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. -
细胞定位
Cell membrane. Secreted. Soluble form found in a number of tissues. - Information by UniProt
-
数据库链接
- Entrez Gene: 966 Human
- Omim: 107271 Human
- SwissProt: P13987 Human
- Unigene: 278573 Human
- Unigene: 709466 Human
- Unigene: 710641 Human
-
别名
- 16.3A5 antibody
- 1F5 antibody
- 1F5 antigen antibody
see all
图片
数据表及文件
-
SDS download
-
Datasheet download
文献 (9)
ab18237 被引用在 9 文献中.
- Cupino TL et al. Stability and bioactivity of chitosan as a transfection agent in primary human cell cultures: A case for chitosan-only controls. Carbohydr Polym 180:376-384 (2018). PubMed: 29103517
- Makkonen KE et al. 6-o- and N-sulfated syndecan-1 promotes baculovirus binding and entry into Mammalian cells. J Virol 87:11148-59 (2013). PubMed: 23926339
- Wickham SE et al. Mapping the Intermedilysin-Human CD59 Receptor Interface Reveals a Deep Correspondence with the Binding Site on CD59 for Complement Binding Proteins C8{alpha} and C9. J Biol Chem 286:20952-62 (2011). Functional Studies ; Human . PubMed: 21507937
- LaChapelle S et al. Intermedilysin-receptor interactions during assembly of the pore complex: assembly intermediates increase host cell susceptibility to complement-mediated lysis. J Biol Chem 284:12719-26 (2009). FRET ; Human . PubMed: 19293153
- Cebecauer M et al. Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells. Biochem Biophys Res Commun 243:706-10 (1998). PubMed: 9500981
- Cinek T & Horejsí V The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases. J Immunol 149:2262-70 (1992). PubMed: 1382093
- Stefanová I et al. GPI-anchored cell-surface molecules complexed to protein tyrosine kinases. Science 254:1016-9 (1991). PubMed: 1719635
- Forsberg UH et al. Gene for human CD59 (likely Ly-6 homologue) is located on the short arm of chromosome 11. Immunogenetics 30:188-93 (1989). PubMed: 2476389
- Stefanová I et al. Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol. Mol Immunol 26:153-61 (1989). PubMed: 2918859