Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Rodent Block M (Biocare Medical) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Target Retrieval Solution pH6.1 (DAKO)
Sample
Mouse Tissue sections (Spleen)
Specification
Spleen
Permeabilization
No
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Antibody Dilutent (DAKO)
Dilution
1/50
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Fixation of spleens were done in 4% paraformaldehyde over nicght at 4¯C. Washed 1 hour in PBS at 4¯C and than stored in 70% EtOH at 4¯C until embedding
The permeablization/ antigen demasking step was performed at 90¯C for 40 minutes using ten fold diluted "Target Retrieval Solution pH6.1"
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Eike Wegener
Verified customer
提交于 Sep 06 2013