Application
Immunocytochemistry
Sample
Mouse Cell (primary murine astrocytes)
Permeabilization
Yes - 0.1% TritonX
Specification
primary murine astrocytes
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 5% goat serum 1mg/ml BSA PBS
Dilution
1/500
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Fluor 488 IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Primary astrocytes isolated from CD44 knockout mice served as negative control.
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提交于 Dec 03 2015