FITC荧光Anti-CD43抗体[MEM-59] (ab21853)
Key features and details
- FITC Mouse monoclonal [MEM-59] to CD43
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
概述
-
产品名称
FITC荧光Anti-CD43抗体[MEM-59]
参阅全部 CD43 一抗 -
描述
FITC荧光小鼠单克隆抗体[MEM-59] to CD43 -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD43. T lymphocytes
-
常规说明
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: PBS, BSA -
Concentration information loading...
-
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
MEM-59 -
同种型
IgG1 -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab21853于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use 1µl for 106 cells.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
---|
Flow Cyt
Use 1µl for 106 cells. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
-
功能
One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. -
组织特异性
Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. -
翻译后修饰
Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 6693 Human
- Omim: 182160 Human
- SwissProt: P16150 Human
- Unigene: 632188 Human
-
别名
- CD 43 antibody
- CD43 antibody
- CD43 antigen antibody
see all
图片
-
Flow Cytometry analysis of human peripheral blood cells labeling CD43 with Anti-CD43 antibody [MEM-59] (FITC) (ab21853).
-
Overlay histogram showing peripheral blood lymphocytes stained with ab21853(red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21853, 0.5 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (2 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab21853 被引用在 2 文献中.
- Tingling JD et al. CD24 expression identifies teratogen-sensitive fetal neural stem cell subpopulations: evidence from developmental ethanol exposure and orthotopic cell transfer models. PLoS One 8:e69560 (2013). Flow Cyt ; Mouse . PubMed: 23894503
- Stracker TH et al. The carboxy terminus of NBS1 is required for induction of apoptosis by the MRE11 complex. Nature 447:218-21 (2007). WB ; Mouse . PubMed: 17429352