Application
Western blot
Sample
Human Cell lysate - whole cell (HCAEC)
Specification
HCAEC
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit IgG, HRP linked whole
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
20 second(s)
Bands
Specific: 45 kDa
Positive control
Skip 1st or 2nd Ab
Additional data
Additional Notes
Lanes 1 & 2: Control HCAEC
Lanes 3 & 4: Treated HCAEC
Lanes 3 & 4: Treated HCAEC
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Aug 10 2005