Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 40% · Temperature: 20°C
Sample
Human Tissue sections (human tonsil)
Specification
human tonsil
Permeabilization
Yes - saponin
Fixative
Paraformaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Jackson Immunoresearch Alexa Fluor 594 AffiniPure
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 594
Dilution
1/150
Additional data
Additional Notes
Unfit for IHC on frozen samples. Non specific staining in the germinal center. Tested also with acetone fixation and no permeabilization with similarly poor results.
Abcam response
Thank you for your valuable feedback. ab101530 has not been previously tested in IHC frozen section. It is possible that this antibody may not be suitable for use in this application. We welcome other Abreviews to determine this.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Jan Misiak
Verified customer
提交于 May 13 2014